Normally synthesized from the essential amino acid glutamate, proline produces proteins like cartilage and collagen. Almost one-third of the amino acids in collagen are proline, making this nonessential amino acid incredibly essential to tissue repair and skin regeneration. Proline production increases during times of soft-tissue trauma, injury, and wound healing, such as muscle or tendon recovery, severe burns, and after surgery. Proline also helps prevent arteriosclerosis and regulate blood pressure by encouraging the walls of the arteries to release fat build-up into the blood, thereby reducing the risk of blockage. By decreasing the pressure built up by these blockages, proline helps lower blood pressure and the risk of heart disease.